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- 1821 — Town of Micanopy is founded
- 1853 — Presbyterian church organized in Micanopy
- 1857 — First Episcopal services held in Micanopy
- 1873 — Presbyterians acquired the lot on Cholokka Blvd. where the Church of the Mediator is located today. Shortly after, the Presbyterians built the wood-frame church that still stands today, now owned by The Episcopal Church.
- 1896 — Episcopalians had a small frame church on the south side of Seminary Street, between Cholokka Blvd. and Division Street
- 1910’s — Episcopal services cease.
- 1920’s — Episcopal church building torn down
- 1960’s — Presbyterian services cease, building on Cholokka St. is vacant
- 1966 — October 16, Episcopal services again conducted in Micanopy, with Archdeacon Fred Yerkes of Jacksonville. The name, Church of the Mediator, is a revival of the name of the earlier church.
- 1983 — Easter Day. Father Les Singleton begins serving at the Church of the Mediator
- 2005 — August. James Gilbert becomes organist and music director
- 2007 — November. Church buys new Kawai grand piano
- 2007 — December 16. Father Les Singleton celebrates his 40th ordination anniversary
- 2013 — March. Church buys new electronic organ and speaker system. (Building is too small for an appropriate pipe-organ)
- 2017 — Father Les Singleton celebrates his 50th ordination anniversary (December 16)
- 2019 — Father Les Singleton retires after nearly 37 years as Vicar. Father George Holston becomes vicar.
- 2020 — Covid19 pandemic results in the cancellation of in-person services. Services are held via video conferencing over the internet.
- 2021 — Palm Sunday. Services resume in the church building with numerous restrictions including modified communion and no coffee hour after church. Over the next year the restrictions are slowly removed.
- 2022 — Spring. Services in person return to pre-pandemic practices. Coffee hour after church resumes.
- 2024 – Organ suffers a power surge and dies. A temporary replacement was made until a 3-manual Viscount organ was installed in 2025.